Spent some time today in the kitchen lab. Created some liquid culture solution and introduced some shiitake culture. I’m spending time lately learning all sorts of smaller techniques that will lead to a well rounded education in the amateur mycology field. Petri dishes with various agar media are next. Some day I’ll actually grow some mushrooms instead of just poking around with cultures. Yesterday I went to Whole Foods and noticed that certain mushrooms were selling to $20 a pound…. needless to say I drooled a little.
Here is a hacked description of today’s project.
100ml water – 1tsp Light Karo Syrup.
Mixed this up 4 times and put them into separate jars.
Covered them with two layers of aluminum foil.
Pressure cooked for 10 minutes. I’m told after 15 minutes of pressure cooking the sugars caramelize, and growth will be slower as a result.
Cooled and then inoculated with some Shiitake mycelium that I had growing on cardboard.
To clean the sharp tool used to cut, I dropped a little denatured alcohol onto the bottom of a shot glas and lit it on fire. Very tiny small blue flame.
To ‘clean’ the spawn, I dipped it in a h2o2 solution – 80ml water – 20ml 30% h2o2.
The Karo syrup solution is now incubating in a 7 gallon bucket with a heat bomb keeping it warm.
Information on the Karo TEK from Mycotopia
Liquid Culture Basics from the Shroomery
An article about the Heat Bomb from 2004 on the Shroomery